Research paper cellulase production

Enzyme production by the selected strains was tested with different culture conditions [ 2728 ].

Cellulase enzyme production ppt

Inferred that under acidic conditions A. This study will help the researcher, to uncover the critical areas of fungal cellulase production to be suitable for industrial implications that many researchers were not able to explore. Considering highest cellulase activity, Paenibacillus sp. Cellulose is mainly degraded by cellulase enzyme which is commonly produced by bacteria and fungi [ 6 ]. Limitation There are two limitations of present study. Nine fungal isolates of seven fungi were randomly selected based on the difference in the plate morphology and were subjected to routine subculturing, preservation and subjected to identification. C1 , Bacillus sp. Carbon sources have been replaced by various substances. Fungi are advantageous as the enzyme production rate is higher compared to other microorganism, more than a few fungi were produced such as Aspergillus niger , Aspergillus fumigates, Alternaria sp. Also, the access to cellulose is more difficult, since it was embedded in other cell wall components, such as hemicellulose and lignin 8. In present study, the isolated strains were characterized by morphological and biochemical analysis [ 19 ] and then screened for their cellulase activity by Congo red iodine solution and DNS test [ 21 , 25 ]. Then DNS was added to the solution to stop the reaction [ 21 ]. All the experiments were conducted in a set of three triplicates and the standard deviation was evaluated for each set, respectively. Results Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose CMC agar plate were identified as cellulase producing bacteria. These media were used for optimize cellulase production.

Therefore, it has become considerable economic interest to develop suitable processes for effective treatment and utilization of cellulose containing wastes as cheap carbon sources [ 5 ]. Effect of different day of incubation on cellulase production under SmF: The optimal media with optimized pH was inoculated with all the five fungal isolates, observations were recorded after 1, 2, 3, 4, 5, 6 and 7 days of incubation.

enzyme production from bacteria

The broth culture Media, pH were filtered off and transfer into the eppendorf tube to centrifuge at 10, rpm for 5 min to take out cell debris. Another study reported Trichoderma viride and Aspergillus niger were a record as cellulase producing species as 1.

Research paper cellulase production

Results Based on experiments, three bacterial strains produced clear transparent zone into carboxymethyl cellulose CMC agar plate were identified as cellulase producing bacteria. Applied carbon sources were xylose, oat spelt xylan, rich bran xylan, starch, carboxymethyl cellulose, cellobiose, wheat bran xylan and chitin. The partially purified enzyme was dialyzed against the phosphate buffer. Thus this study will be helpful for the handmade paper industries at large. During the study, under in vitro condition five fungal isolates Cladoporium cladosproides, Alternaria alternata, Penicillium chrysogenium, Helminthosporium cyclops and Aspergillus fumigatus are used for production of cellulase enzyme. Inoculum preparation and cellulase production: Uniform spore suspension prepared by mixing spores vigorously and their spore density was measured using Systronics UV-VIS spectrophotometer at nm. To characterize the purified enzyme specificity, we initially use qualitative tests like CMC test and filter paper degradation test. C1 , Bacillus sp. During in vitro optimisation studies for the days of incubation days for five different isolates Cladosporium sp. Considering highest cellulase activity, Paenibacillus sp. A microscopic examination revealed that the isolated strain C1 and C2 was rod shaped and found to be gram positive where as C3 strain was short rod in shape and found to be gram negative. Effect of media on cellulase production under SmF: Three different types of culture media were used as Sabouraud dextrose broth, CMC broth and semi-synthetic czapk dox broth. Fungi are advantageous as the enzyme production rate is higher compared to other microorganism, more than a few fungi were produced such as Aspergillus niger , Aspergillus fumigates, Alternaria sp.

C2 and Aeromonas sp. In vitro production of cellulase enzyme by submerged fermentation and thereby in vitro optimisation of different parameters, for better cultivation and production process through submerged fermentation. Fungal cellulase enzyme were related to industrial implications with special reference to hand made a paper industry that has been performed to increase the yield of cellulase production of recipients strain to rise.

cellulase plate assay
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In vitro Optimisation of Fungal Cellulase Production from Fruit Waste for Handmade Paper Industries